Key words: Listeria monocytogenes, poultry, processing plant, evisceration. ABSTRACT . Listeria antisera (Denka Seiken, Tokyo, Japan), accord- ing to the . Caracterização feno e genotípica de cepas de Listeria monocytogenes isoladas They were also serotyped (Denka Seiken, Japan), and sub–typed by PFGE. Antisera for the typing of Listeria Group O and H antigens. Aids public health and food testing laboratories in identifying sources of infection/contamination to.

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Listeriosis has been mainly reported by those countries, with few or even no reports from Africa, Asia and South America. National Center for Biotechnology InformationU.

Antigen components of each L. Because serotype reactions depend on the quality of the antisera used, which in turn depends on which standardized strains and antigen preparation listerka are chosen, the likelihood of inaccurate or inconsistent assignment of serotype rises when laboratories prepare their own antisera, especially for nonclinical isolates i. Antimicrobial resistance of Listeria monocytogenes.

Identification of mixed-serotype cultures. Similarly, to directly compare H-antigen determination by weiken ELISA method to that by the slide agglutination method, isolates initially were subcultured three times on BHI motility agar, as recommended in listefia slide agglutination kit protocol, to increase the proportion of fully flagellated cells and thereby render a robust reaction with the appropriate H-factor antisera.

Combined ribotyping and random multiprimer DNA analysis to probe the population structure of Listeria monocytogenes. Prior to serotype determination, single colonies of each strain were inoculated onto brain heart infusion BHI Difco motility plates containing 0.


It was observed that the spatial or temporally unrelated strains exhibited similar PFGE profiles, indicating a possible clonal relationship among them. One hundred one L. Isolation and detection of Listeria monocytogenes using fluorogenic and chromogenic substrates for phosphatidylinositol-specific phospholipase C. Since the titers of the antisera vary on a lot-to-lot basis, the dilution levels used with each kit must be optimized.

Wesley RM Coleslaw 4b 4b I. Journal List J Clin Microbiol v.

The two methods were performed independently by two different laboratories. Finally, it is known that the cases documented here seikej not represent the true incidence in the community, especially with regard to perinatal infection, once many laboratories of the region covered by this study do not often send the isolates for further characterization by the Regional Lab.

In addition, mixed-serotype cultures of L. The ELISA serotyping protocol described in this work is a means to attempt to rectify the ambiguities and intra- and interlaboratory variability inherent to the traditional serotyping method, while at the same time allowing laboratories to perform more analyses with significant cost and time savings.

We have adapted a commercially available set of Seike.

DENKA SEIKEN Co.,Ltd. | Salmonella

Therefore, a more accurate study of such infection is necessary in our country. All subsequent steps were performed at room temperature. Initial comparisons of the serotyping methods using L. Molecular subtyping to detect human listeriosis clusters. Serotyping of Listeria monocytogenes and related species. Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR.


Fragment patterns were analyzed according to Tenover et al. The use of a commercially prepared set of antisera and a semiquantitative ELISA format greatly reduce the variability of antiserum quality as well as the inconsistencies in judgement associated with weakly aeiken antigen-antiserum combinations. For each strain-antiserum combination, duplicate wells containing cell suspensions incubated without primary antisera and listeriz incubated without cell suspensions were included as negative controls.

Manufacturer of diagnostic reagents and vaccines,DENKA SEIKEN | Others

The discrepancy was investigated by developing a serogroup-specific colony immunoblot method, which could distinguish these serogroups by differential staining Fig. Isolates were confirmed as L. Nevertheless, these data were of particular concern since emergence and dissemination of antibiotic resistance in clinical isolates of L.

As with the slide agglutination method, serotype assignment using the ELISA method is a three-step process. The thirteen isolates belonged to three serotypes: Services on Demand Journal. An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk.

Such strains would be indistinguishable from serotype 4b.