DS 29190 PDF

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Just prior to transfection, the medium was removed and replaced with 4 ml of the fresh complete medium.

To test 29109 hypothesis, we assessed the interaction of caveolin with Src family tyrosine kinases in vitro using i baculovirus-expressed Src proteins c-Src and v-Src and ii bacterially expressed caveolin fusion proteins. Get an immediate offer. Co-expression with caveolin dramatically suppressed the tyrosine kinase activity of c-Src as measured via an immune complex kinase assay.

Pdyn (prodynorphin) | Gene Report | BioGPS

The construction, expression, and purification of GST-caveolin 1 fusion proteins were as we described previously 2425 Effects of caveolin peptides on the auto-activation of Fyn, a closely related Src family tyrosine kinase. Horseradish peroxidase-conjugated secondary antibodies 1: Issues regarding compatibility should be resolved before purchasing. Be the first to write a review.

We provide a simple no-nonsense returns policy: Expedited Delivery International Priority Shipping. Caveolin-derived Synthetic Peptides Caveolin peptides were designed based on the protein sequence of the N-terminal domain of canine caveolin. After transfer to nitrocellulose, Western blot analysis was performed with an anti-Src mAb probe 1: Email to friends Share on Facebook – opens in a new window or tab Share on Twitter – opens in a new window or tab Share on Pinterest – opens in a new window or tab.


Using either Triton-based methods 121314151617 or detergent-free methods 181920it has been demonstrated that caveolin co-purifies with cytoplasmic lipid-modified signaling molecules. No additional import charges on delivery.

Briefly, phosphorylated protein bands were digitized by high resolution optical scanning; volumetric integration of signal intensities was carried out using ImageQuant Software Fast Scan; Molecular Dynamics. The expression of Src tyrosine kinases was monitored by immunoblot analysis with a mAb that recognizes the conserved extreme N-terminal of Src.


The numbers at end points reflect their exact amino acid position within caveolin. Primary antibodies were detected using distinctly tagged fluorescent secondary antibodies: Casanova, Massachusetts General Hospital Kunxin Luo Whitehead Institute and Dr. As shown in Fig.

A brand-new, unused, unopened and undamaged item in original retail packaging where packaging is applicable. First, it is required to form multivalent homo-oligomers of caveolin. Get the item you ordered or your money back. Peptide 2 encodes residues of caveolin that contain caveolin binding activity as we have shown in Fig.

Representative APR variable Note that peptides 3 and 4 have no effect, indicating that the complete region of caveolin peptide 2 is required for inhibiting the auto-activation of c-Src. Submit your work to JBC.


Co-expression of c-Src and Caveolin in Mammalian T Cells Untagged caveolin and c-Src were co-expressed in T cells by co-transfection using a modified calcium phosphate precipitation procedure as described previously We do not yet know the mechanism by which caveolin-derived peptides inhibit Src family kinases.

Glenney for monoclonal antibodies directed against caveolin; Dr. Arhodamine-conjugated for c-Src; Bfluorescein-conjugated for caveolin; and Csuperposition of the fluorescent images of A and B.

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Note that although both immunoprecipitates contain equivalent amounts of c-Src top panelco-expression with caveolin prevents the auto-phosphorylation of c-Src middle panel. Interestingly, when peptide 2 was divided into two peptides peptides 3 and 4; Fig. You’ll be in good company. However, it is not clear from our experiments whether full-length caveolin can perform this function in vivo. However, in both reports, the investigators used much higher concentrations of peptides, such as 0.

The effects of peptide 1 Apeptide 2 Band peptides 3 and 4 C are shown. Caveolin, a kDa integral membrane protein, is a principal structural and regulatory component of caveolae membranes.

Journal of Lipid Research.