Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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Do you have any advice? The trick is to leave them in just long enough. Las modificaciones de este procedimiento se describen en detalle en Johansen et al. If that doesn’t help, please let us know. That is the volume I prepare each time, usually enough for two or three prep. You will only be able to see the first 20 seconds. Agradecemos a cromosomaz Sra.

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Unable to load video. This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect? Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas. Repita los pasos 4. Skip to content Biology. My thought is that they may be getting flushed out during my transfers.


Fill out the form below to receive a free trial or learn more about access: Fill out the form below to receive a free trial or learn more about access:. If the problem continues, please let us know and politwnicos try to help.

Click here for the english version. Fluorescent in situ Hybridization on Mitotic Chromosomes of Politwnicos. Please recommend JoVE to your librarian.

Lugar pesos g en el cubreobjetos de las diapositivas. If you are seeing few salivary gland chromosome sets you may be losing them when you squash. A squash that is too rapid or poolitenicos may also cause the chromosomes to float away.

Cromosomas politénicos

cromosomaas Is it the PFA? Warm to disperse the Triton X You are right about the Triton. Caliente para dispersar a la Triton X y su uso dentro de una hora. If you leave the glands in too long they will fall apart. Please sign in or create an account. Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos.


Your institution must subscribe to JoVE’s Biology section to access this content. For other languages click here. When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present.

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Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you cromosomxs.

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