ABSTRACT. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Plant Adhatoda vasica L. Nees (Acanthaceae) commonly known as Malabar nut is an evergreen two – three m tall shrub, sometime used as. Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the .

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Vasicine acetate showed antimycobacterial activity [ 15 ]. Journal of Natural Products. Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ]. Published online Jan International Journal of Cancer. BHT is used as standard of antioxidant. National Center if Biotechnology InformationU.

Handbook of Ayurvedic Medicinal Plants. Balachandran2 S.

The free radical scavenging activity was calculated as follows:. Ray of hope for cancer patients. Negative control was prepared using respective solvents. The antifungal agent Ketoconazole for fungi and antibacterial agent Streptomycin for bacteria were included in the assays as positive controls. The plant contains alkaloids such as Vasicine, vasicinone, deoxyvasicine, vasicol, adhatodinine, and vasicinol [ 12 ].

The solutions were filtered through a membrane filter pore size 0. Vasicine acetate was obtained by acetylation of Vasicine. Effect of Adhatoda vasica massage in pyorrhoea. Materials and Methods 2. Essential oils of the leaves of A. It was then distilled on a water bath and the residue was washed with little n-hexane. A voucher specimen No. Kulkarni [ 24 ] reported that A. Vasicine acetate showed moderate antibacterial activity compared antimicrboial Vasicine. Shade dried and coarsely powdered leaves of A.


Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi. The plant was identified by Dr. Nees Acanthaceaeknown commonly as Malabar nut tree, is a shrub growing throughout the Indian peninsula. DPPH 2,2-diphenylpicrylhydrazyl antimicrobil scavenging activity of Vasicine acetate was determined based on the method of Wang et al. Indian Journal of Natural Products and Resources.

Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m. The required concentrations, The following Gram negative, Gram positive bacteria, clinical isolates, and fungi actkvity used for the experiment: B revised New Delhi, India: Journal of Obstetrics and Gynaecology of India.

Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis Lep. International Journal of Green Pharmacy.

The extract was filtered through Whatman No. Conflict of Interests The authors declare that there is no conflict kf interests regarding the publication of this paper.

Acanthaceae is used in the indigenous system of medicine in India. The physical and spectroscopic data were compared with those reported in the literature [ 15 ].

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Other constituents include vitamin C, saponins, flavonoids as well as steroids, and fatty acids [ 13 ]. The authors declare that there is no conflict of interests regarding the publication of this paper. Cytotoxicity of each sample was expressed as IC 50 value. Minimum Inhibitory Concentration MIC Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].


Open in a separate antimicfobial. Ignacimuthu and Shanmugam [ 15 ] have reported the antitubercular activity of Vasicine acetate isolated from A. Natural products still remain the most important source for discovery of new and potential drug molecules.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Antibacterial activity of adhatoda vasica leaf extract

The column was eluted with chloroform and chloroform: Medicinal plants are important sources of practical drugs for people throughout the year. The combined chloroform extract was washed with water, dipped, zdhatoda dried over anhydrous sodium sulphate.

The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ].

The MIC for bacteria was determined as the lowest concentration of the compound inhibiting the visual growth of the test cultures on the agar plate. The physical and spectroscopic data were compared with those reported in the literature [ 21 adhatora, 22 ]. All melting point values are uncorrected and adhatoa by open capillary method on a heating block Instrument.